pdestRG5273Neo
(Plasmid
#26390)
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Purpose(Empty Backbone)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 26390 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBS
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Vector typeBacterial Expression, Worm Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Growth instructionsccdB resistant bacteria ex: DB3.1 E. coli
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNone
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SpeciesC. elegans (nematode)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
extremely slow growing.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pdestRG5273Neo was a gift from Denis Dupuy (Addgene plasmid # 26390 ; http://n2t.net/addgene:26390 ; RRID:Addgene_26390) -
For your References section:
An antibiotic selection marker for nematode transgenesis. Giordano-Santini R, Milstein S, Svrzikapa N, Tu D, Johnsen R, Baillie D, Vidal M, Dupuy D. Nat Methods. 2010 Aug 22. ():. 10.1038/nmeth.1494 PubMed 20729841
Map uploaded by the depositor.
