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Addgene

pQCXIR (w498-1)
(Plasmid #26287)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26287 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pQCXIP
  • Backbone manufacturer
    Clontech
  • Backbone size (bp) 7072
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    RFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    TOP10F', 37oC
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    no insert
  • Alt name
    Red Fluorescent Protein 2

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Age I (not destroyed)
  • 3′ cloning site Bam HI (not destroyed)
  • 5′ sequencing primer CMVforw
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    DsRed2 cDNA was derived from pIRES DsRed 2 plasmid (Clontech)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A cDNA or miRNA can be inserted downstream of the CMV promoter. There is an IRES with the red fluorescent protein downstream so the transduced cells will be red.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQCXIR (w498-1) was a gift from Eric Campeau (Addgene plasmid # 26287 ; http://n2t.net/addgene:26287 ; RRID:Addgene_26287)
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