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543-p3E-2A-mcherrypA
(Plasmid #26031)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26031 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p3E
  • Backbone size w/o insert (bp) 2946
  • Vector type
    Entry vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mCherry
  • Insert Size (bp)
    710

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site attR2 (not destroyed)
  • 3′ cloning site attL3 (not destroyed)
  • 5′ sequencing primer M13pUC-fwd
  • 3′ sequencing primer M13rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

"p3E" refers to 3' entry clones flanked by R2-L3.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    543-p3E-2A-mcherrypA was a gift from Nathan Lawson (Addgene plasmid # 26031 ; http://n2t.net/addgene:26031 ; RRID:Addgene_26031)

  • For your References section:

    A truncation allele in vascular endothelial growth factor c reveals distinct modes of signaling during lymphatic and vascular development. Villefranc JA, Nicoli S, Bentley K, Jeltsch M, Zarkada G, Moore JC, Gerhardt H, Alitalo K, Lawson ND. Development. 2013 Apr;140(7):1497-506. doi: 10.1242/dev.084152. Epub 2013 Mar 5. 10.1242/dev.084152 PubMed 23462469
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