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Addgene

476 p5E-mcs
(Plasmid #26029)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26029 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p5E
  • Backbone size (bp) 2810
  • Vector type
    Entry vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site attL1 (not destroyed)
  • 3′ cloning site attR2 (not destroyed)
  • 5′ sequencing primer M13pUC-fwd
  • 3′ sequencing primer M13rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

"p5E" refers to 5' entry clones flanked by L4-R1

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    476 p5E-mcs was a gift from Nathan Lawson (Addgene plasmid # 26029 ; http://n2t.net/addgene:26029 ; RRID:Addgene_26029)

  • For your References section:

    Robust Identification of Developmentally Active Endothelial Enhancers in Zebrafish Using FANS-Assisted ATAC-Seq. Quillien A, Abdalla M, Yu J, Ou J, Zhu LJ, Lawson ND. Cell Rep. 2017 Jul 18;20(3):709-720. doi: 10.1016/j.celrep.2017.06.070. 10.1016/j.celrep.2017.06.070 PubMed 28723572
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