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Addgene

475 p5EfliEBSbasP
(Plasmid #26027)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26027 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p5E
  • Backbone size w/o insert (bp) 2858

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    fli1a intron 1
  • Species
    D. rerio (zebrafish)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

"p5E" refers to 5' entry clones flanked by L4-R1

There are a few mismatches between depositor's sequence and Addgene's sequence. The changes should not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    475 p5EfliEBSbasP was a gift from Nathan Lawson (Addgene plasmid # 26027 ; http://n2t.net/addgene:26027 ; RRID:Addgene_26027)

  • For your References section:

    A truncation allele in vascular endothelial growth factor c reveals distinct modes of signaling during lymphatic and vascular development. Villefranc JA, Nicoli S, Bentley K, Jeltsch M, Zarkada G, Moore JC, Gerhardt H, Alitalo K, Lawson ND. Development. 2013 Apr;140(7):1497-506. doi: 10.1242/dev.084152. Epub 2013 Mar 5. 10.1242/dev.084152 PubMed 23462469
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