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Addgene

pMXs-Ms-L-Myc
(Plasmid #26023)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26023 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMXs
  • Backbone manufacturer
    Dr. Toshio Kitamura of the University of Tokyo
  • Backbone size w/o insert (bp) 6340
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    L-Myc
  • Alt name
    L-myc; Lmyc1; Lmyc-1; Mycl1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1107
  • Mutation
    None
  • Entrez Gene
    Mycl (a.k.a. L-myc, Lmyc-1, Lmyc1, Mycl1, bHLHe38)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site attR1 (not destroyed)
  • 3′ cloning site attR2 (not destroyed)
  • 5′ sequencing primer pMX-S1811 (GAC GGC ATC GCA GCT TGG ATA CAC)
  • 3′ sequencing primer pMX-TgAS (GAC ATG GCC TGC CCG GTT ATT ATT)
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pMXs is from Dr. Toshio Kitamura of the University of Tokyo, the Institute of Medical Science. If you use this plasmid in a paper, please cite: Retrovirus-mediated gene transfer and expression cloning: powerful tools in functional genomics. Exp Hematol. 2003 Nov;31(11):1007-14. Kitamura T, Koshino Y, Shibata F, Oki T, Nakajima H, Nosaka T, Kumagai H.
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMXs-Ms-L-Myc was a gift from Shinya Yamanaka (Addgene plasmid # 26023 ; http://n2t.net/addgene:26023 ; RRID:Addgene_26023)

  • For your References section:

    Promotion of direct reprogramming by transformation-deficient Myc. Nakagawa M, Takizawa N, Narita M, Ichisaka T, Yamanaka S. Proc Natl Acad Sci U S A. 2010 Jul 26. ():. 10.1073/pnas.1009374107 PubMed 20660764
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