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pSIN4-EF2-ABCG2-IRES-Neo
(Plasmid #25983)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 25983 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSIN-EF2-MCS-IRES-Neo
  • Backbone manufacturer
    James Thomson
  • Backbone size w/o insert (bp) 6500
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ATP-binding cassette, sub-family G (WHITE), member 2, variant of isoform 2
  • Alt name
    ABCG2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1968
  • Mutation
    None
  • GenBank ID
    AAH92408.1
  • Entrez Gene
    ABCG2 (a.k.a. ABC15, ABCP, BCRP, BCRP1, BMDP, CD338, CDw338, CDw388, EST157481, GOUT1, MRX, MXR, MXR-1, MXR1, UAQTL1)
  • Tags / Fusion Proteins
    • EF1-apha promoter (N terminal on backbone)
    • IRES-Neo (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR I (unknown if destroyed)
  • 3′ cloning site BamH I (unknown if destroyed)
  • 5′ sequencing primer EF-1a-F
  • 3′ sequencing primer IRES-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that the backbone of this plasmid is larger than the reported 6.5kb (see attached diagnostic digest).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSIN4-EF2-ABCG2-IRES-Neo was a gift from Ren-he Xu (Addgene plasmid # 25983 ; http://n2t.net/addgene:25983 ; RRID:Addgene_25983)

  • For your References section:

    Lack of ABCG2 expression and side population properties in human pluripotent stem cells. Zeng H, Park JW, Guo M, Lin G, Crandall L, Compton T, Wang X, Li XJ, Chen FP, Xu RH. Stem Cells. 2009 Oct . 27(10):2435-45. 10.1002/stem.192 PubMed 19670287
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