pLX304
(Plasmid #25890)

• Purpose: (Empty Backbone) 3rd generation lentiviral vector
• Depositing Lab: David Root
• Publication: Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. doi: 10.1038/nmeth.1638

Backbone

• Vector backbone: pLKO
• Backbone size (bp): 9377
• Vector type: Mammalian Expression, Lentiviral ; Gateway Destination vector
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): ccdB Survival
• Growth instructions: ccdB resistant bacteria. 30C for liquid culture. 37C for plates.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None
• Tag / Fusion Protein:
  • V5 (C terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: WPRE-R

Resource Information

• Supplemental Documents:
  • pLX Gateway manual
• Addgene Notes:
  • Addgene Diagnostic Digest for 25890
  • Addgene Diagnostic Digest
• Articles Citing this Plasmid:
  • 121 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There is a C-terminal V5 tag followed by a Stop codon directly after the Gateway sequence.

Information for Cloning Grade DNA (Catalog # 25890-DNA.cg) ( Back to top)

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

• Amount: 2 µg
• Guaranteed Concentration: 100 ng/µl +/- 5 ng/µl
• Pricing: $105 USD
• Storage: DNA can be stored at 4℃ (short term) or -20℃ (long term).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here