pCS2 mouse twisted gastrulation
(Plasmid #25778)

• Depositing Lab: Edward De Robertis
• Publication: Mouse Twisted Gastrulation (unpublished)

Backbone

• Vector backbone: pCS2+
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 4095
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: twisted gastrulation
• Species: M. musculus (mouse)
• Insert Size (bp): 669
• Entrez Gene: Twsg1 (a.k.a. Tsg, Twg, AW552143, D17Ertd403e, 1810013J15Rik, 9030422N06Rik)
• Tags / Fusion Proteins:
  • chordin signal peptide (N terminal on backbone)
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: T7

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Xenopus chordin signal peptide N-epitope. Will homo-dimerize, binds covalently to Chordin and is recognized by R&D monoclonal anti-TSG antibody MAB756. Prevents the making of Ternary complex as published in Oelgeschlager M, et al., 2003b. Purified protein is readily ventralizing when injected in blastocoel.