pEN_TGmiR_Luc-A
(Plasmid #25765)

• Purpose: Entry vector withTRE promoter driving control Luciferase miR30-based shRNA with GFP coexpression.
• Depositing Lab: Iain Fraser
• Publication: Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 103(37):13759-64.

Backbone

• Vector backbone: pENTR1A-Gent
• Backbone manufacturer: ATCC 10326362
• Backbone size w/o insert (bp): 4990
• Vector type: Entry vector

Growth in Bacteria

• Bacterial Resistance(s): Gentamicin, 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Growth instructions: Top10
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Luciferase miR-shRNA
• Tag / Fusion Protein:
  • GFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: attL1 (not destroyed)
• 3′ cloning site: attL2 (not destroyed)
• 5′ sequencing primer: pCEP-fwd
• 3′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: TRE promoter- David Anderson, Caltech; miR30- Greg Hannon, CSHL

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Entry vector with TRE-driven luciferase miR-shRNA and co-expressed GFP

Luciferase shRNA sequence is - 5'- CCCGCCTGAAGTCTCTGATTAATAGTGAAGCC ACAGATGTATTAATCAGAGACTTCAGGCGGT