pEN_TmiRc3
(Plasmid #25748)

• Purpose: (Empty Backbone) Entry vector for cloning miR30-based shRNA driven by TRE promoter.
• Depositing Lab: Iain Fraser
• Publication: Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 103(37):13759-64.

Backbone

• Vector backbone: pENTR1A-Gent
• Backbone manufacturer: ATCC 10326362
• Backbone size (bp): 4608
• Vector type: Entry vector

Growth in Bacteria

• Bacterial Resistance(s): Gentamicin, 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DB3.1
• Growth instructions: DB3.1 (ccdB survival)
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: attL1 (not destroyed)
• 3′ cloning site: attL2 (not destroyed)
• 5′ sequencing primer: pCEP-fwd
• 3′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: TRE promoter- David Anderson, Caltech; miR-30- Greg Hannon, CSHL
• Articles Citing this Plasmid:
  • 11 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

shRNA expression; miR30-based topology; TRE Pmin promoter; Entry vector backbone; +ccdB in parent; Multiple unique sites d/s of TRE