pSLIK-Neo-TGmiR-Luc
(Plasmid #25745)

• Purpose: Control lentiviral vector with Tet-based inducible expression of Luciferase miR30-based shRNA and GFP, constitutive Neomycin resistance gene coexpression.
• Depositing Lab: Iain Fraser
• Publication: Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 103(37):13759-64.

Backbone

• Vector backbone: pSLIK
• Backbone manufacturer: Iain Fraser
• Backbone size w/o insert (bp): 13217
• Vector type: Mammalian Expression, Lentiviral, RNAi

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Growth instructions: Top10
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Luciferase miR-shRNA
• Tag / Fusion Protein:
  • GFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BfuA1 (not destroyed)
• 3′ cloning site: BfuA1 (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: Neo-R, hUBCpro-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: rtTA- Atze Das, University of Amsterdam; TRE promoter- David Anderson, Caltech; miR30- Greg Hannon, CSHL

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Lentivirus; Inducible TRE driven GFP-shRNA; Constitutive Ubi-c driven rtTA3-IRES-Neo

Luciferase shRNA sequence is - 5'- CCCGCCTGAAGTCTCTGATTAATAGTGAAGCC ACAGATGTATTAATCAGAGACTTCAGGCGGT