pGli3a
(Plasmid
#25733)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 25733 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepBluescript II (KS)
- Backbone size w/o insert (bp) 2958
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGli3
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SpeciesM. musculus (mouse)
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Insert Size (bp)800
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Entrez GeneGLI3 (a.k.a. ACLS, GCPS, GLI3-190, GLI3FL, PAP-A, PAPA, PAPA1, PAPB, PHS, PPDIV)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Insert containing the entire ZnF domain
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGli3a was a gift from C.C. Hui (Addgene plasmid # 25733 ; http://n2t.net/addgene:25733 ; RRID:Addgene_25733) -
For your References section:
A mouse model of greig cephalopolysyndactyly syndrome: the extra-toesJ mutation contains an intragenic deletion of the Gli3 gene. Hui CC, Joyner AL. Nat Genet. 1993 Mar . 3(3):241-6. 10.1038/ng0393-241 PubMed 8387379
Map uploaded by the depositor.
![](https://media.addgene.org/data/easy-thumbnails/data/89/87/4b85e7c0-af63-11e0-90fe-003048dd6500.jpeg.940x940_q85_autocrop.png)