pCDNA3-HA-mMcl1
(Plasmid #25714)

• Depositing Lab: Roger Davis
• Publication: Morel et al Mol Cell Biol. 2009 Jul . 29(14):3845-52.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: invitrogen
• Backbone size w/o insert (bp): 5446
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mMCL1
• Species: M. musculus (mouse)
• Insert Size (bp): 1270
• GenBank ID: NM_008562
• Entrez Gene: Mcl1 (a.k.a. Gm52627, Mcl-1)
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (destroyed during cloning)
• 3′ cloning site: EcoRI (destroyed during cloning)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Hsin-Fang Yang-Yen "Stabilization and Enhancement of the Antiapoptotic Activity of Mcl-1 by TCTP"
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry