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YCPlac33-sGFP-VRG4
(Plasmid #25447)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 25447 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    YCPlac33
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Synthetic Green Fluorescent Protein - VRG4
  • Alt name
    sGFP-VRG4
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    2985
  • Tag / Fusion Protein
    • GFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer gcgggcagtgagcgcaacgcaattaa
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    YCPlac33-sGFP-VRG4 was a gift from Benjamin Glick (Addgene plasmid # 25447 ; http://n2t.net/addgene:25447 ; RRID:Addgene_25447)

  • For your References section:

    Golgi maturation visualized in living yeast. Losev E, Reinke CA, Jellen J, Strongin DE, Bevis BJ, Glick BS. Nature. 2006 Jun 22. 441(7096):1002-6. 10.1038/nature04717 PubMed 16699524
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