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Addgene

pMA2867
(Plasmid #25439)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 25439 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMA2767
  • Backbone manufacturer
    unpublished
  • Backbone size w/o insert (bp) 6700
  • Vector type
    Mammalian Expression, Lentiviral, RNAi
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mCherry-miR155-lacZalpha
  • Species
    M. musculus (mouse); Discosoma-mouse-E. coli
  • Insert Size (bp)
    1300
  • GenBank ID
    AY096003
  • Entrez Gene
    Mir155hg (a.k.a. Bic, Gm26667)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (destroyed during cloning)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Clontech, Invitrogen

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMA2867 was a gift from Mikhail Alexeyev (Addgene plasmid # 25439 ; http://n2t.net/addgene:25439 ; RRID:Addgene_25439)

  • For your References section:

    A retro-lentiviral system for doxycycline-inducible gene expression and gene knockdown in cells with limited proliferative capacity. Alexeyev MF, Fayzulin R, Shokolenko IN, Pastukh V. Mol Biol Rep. 2010 Apr . 37(4):1987-91. 10.1007/s11033-009-9647-7 PubMed 19655272
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