pCDNA3.1-hMcl-1(S159A)
(Plasmid #25374)

• Depositing Lab: Roger Davis
• Publication: Morel et al Mol Cell Biol. 2009 Jul . 29(14):3845-52.

Backbone

• Vector backbone: pcDNA3.1D V5-His-TOPO
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5514
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: hMCL1(S159A)
• Alt name: MCL1
• Species: H. sapiens (human)
• Insert Size (bp): 1100
• Mutation: changed serine 159 to alanine
• GenBank ID: NM_021960
• Entrez Gene: MCL1 (a.k.a. BCL2L3, EAT, MCL1-ES, MCL1L, MCL1S, Mcl-1, TM, bcl2-L-3, mcl1/EAT)
• Tag / Fusion Protein:
  • V5 and His tag (C terminal on backbone)

Cloning Information

• Cloning method: TOPO Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Ulrich Maurer-"Glycogen Synthase Kinase-3 Regulates Mitochondrial Outer Membrane Permeabilization and Apoptosis by Destabilization of MCL-1"
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There are a few mismatches between the sequence provided and the existing NCBI sequence, specifically - E168 deletion and D172N D173E mutations.
The construct works fine and the mismatches are probably due to outdated reference sequence in NCBI.

The tags on this plasmid are not in frame with hMcl-1.