EF.STAT3C.Ubc.GFP
(Plasmid #24983)

• Purpose: 3rd generation lentiviral expression of constitutively active Stat3
• Depositing Lab: Linzhao Cheng
• Publication: Hillion et al Cancer Research 68, 10121, December 2008.

Backbone

• Vector backbone: cFUGW
• Backbone size w/o insert (bp): 11500
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: EGFP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: STAT3
• Species: H. sapiens (human)
• Insert Size (bp): 2314
• Mutation: A662C, N664C (constitutively active)
• GenBank ID: NM_139276.2
• Entrez Gene: STAT3 (a.k.a. ADMIO, ADMIO1, APRF, HIES)
• Tag / Fusion Protein:
  • FLAG (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PacI (destroyed during cloning)
• 3′ cloning site: PacI (destroyed during cloning)
• 5′ sequencing primer: EF1a-forward
• 3′ sequencing primer: hUBCpro-R

Resource Information

• Articles Citing this Plasmid:
  • 43 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

A V667L mutation is present but not known to affect function.

Expression of EGFP is driven by the human ubiquitin promoter. See Addgene plasmid 14883: FUGW for more information on the empty vector.