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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 24982 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonecFUGW
- Backbone size w/o insert (bp) 11500
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Growth instructionsStbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHES1
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SpeciesH. sapiens (human)
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Insert Size (bp)800
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MutationDNA-binding domain disrupted by E43A, K44A, R47A mutations.
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Entrez GeneHES1 (a.k.a. HES-1, HHL, HRY, bHLHb39)
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Tag
/ Fusion Protein
- FLAG (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer EF1a-forward
- 3′ sequencing primer hUBCpro-R (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
EF.deltaBHES1.Ubc.GFP was a gift from Linzhao Cheng (Addgene plasmid # 24982 ; http://n2t.net/addgene:24982 ; RRID:Addgene_24982) -
For your References section:
HES1 inhibits cycling of hematopoietic progenitor cells via DNA binding. Yu X, Alder JK, Chun JH, Friedman AD, Heimfeld S, Cheng L, Civin CI. Stem Cells. 2006 Apr . 24(4):876-88. 10.1634/stemcells.2005-0598 PubMed 16513761