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Addgene

EF.deltaBHES1.Ubc.GFP
(Plasmid #24982)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24982 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    cFUGW
  • Backbone size w/o insert (bp) 11500
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HES1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    800
  • Mutation
    DNA-binding domain disrupted by E43A, K44A, R47A mutations.
  • Entrez Gene
    HES1 (a.k.a. HES-1, HHL, HRY, bHLHb39)
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EF.deltaBHES1.Ubc.GFP was a gift from Linzhao Cheng (Addgene plasmid # 24982 ; http://n2t.net/addgene:24982 ; RRID:Addgene_24982)
  • For your References section:

    HES1 inhibits cycling of hematopoietic progenitor cells via DNA binding. Yu X, Alder JK, Chun JH, Friedman AD, Heimfeld S, Cheng L, Civin CI. Stem Cells. 2006 Apr . 24(4):876-88. 10.1634/stemcells.2005-0598 PubMed 16513761