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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 17624 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonecFUGW
- Backbone size w/o insert (bp) 11500
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Vector typeMammalian Expression, Lentiviral
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Selectable markersGFP
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameHES1
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Alt namehHES1
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Alt nameHes-1
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SpeciesH. sapiens (human)
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Insert Size (bp)850
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Entrez GeneHES1 (a.k.a. HES-1, HHL, HRY, bHLHb39)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer EF-1a Forward (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
hHES1 driven by the EF-1a promoter and a GFP reporter driven by the ubiquitin C (Ubc) promoter.
Recombinant LVs were produced by transient transfection of the transducing vector into 293T cells with two packaging vectors: pMD.G, a plasmid expressing the VSV-G envelope gene, and pCMVDeltaR8.91, a plasmid expressing the HIV-1 gag/pol, tat, and rev genes.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
EF.hHES1.Ubc.GFP was a gift from Linzhao Cheng (Addgene plasmid # 17624 ; http://n2t.net/addgene:17624 ; RRID:Addgene_17624) -
For your References section:
HES1 inhibits cycling of hematopoietic progenitor cells via DNA binding. Yu X, Alder JK, Chun JH, Friedman AD, Heimfeld S, Cheng L, Civin CI. Stem Cells. 2006 Apr . 24(4):876-88. 10.1634/stemcells.2005-0598 PubMed 16513761