pCFJ66
(Plasmid
#24981)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 24981 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDONR221
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 2544
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Vector typeMultisite Gateway
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCbr-unc-119 Punc-122::GFP
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SpeciesC. elegans (nematode); C. briggsae
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Insert Size (bp)4300
Cloning Information
- Cloning method Gateway Cloning
- 5′ cloning site attB1 (destroyed during cloning)
- 3′ cloning site attB2R (destroyed during cloning)
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCFJ66 was a gift from Erik Jorgensen (Addgene plasmid # 24981 ; http://n2t.net/addgene:24981 ; RRID:Addgene_24981) -
For your References section:
Targeted gene deletions in C. elegans using transposon excision. Frokjær-Jensen C, Davis MW, Hollopeter G, Taylor J, Harris TW, Nix P, Lofgren R, Prestgard-Duke M, Bastiani M, Moerman DG, Jorgensen EM. Nat Methods. 2010 Apr 25. ():. 10.1038/nmeth.1454 PubMed 20418868