Puro 2.0
(Plasmid #24970)

• Purpose: (Empty Backbone)
• Depositing Lab: Tyler Jacks
• Publication: Young et al Proc Natl Acad Sci U S A. 2010 Jun 1. 107(22):10184-9.

Backbone

• Vector backbone: Puro 2.0
• Backbone size (bp): 7705
• Vector type: Mammalian Expression, Lentiviral, RNAi
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HpaI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: MSCV-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This vector was derived from pSICO-pgk Puro, and no longer contains the loxp sites. Therefore, Puro 2.0 allows constitutive shRNA expression in mammalian cells. The shRNA coding oligos have to be cloned into the HpaI and XhoI restriction sites. Oligo design information can be found on the author's map or on the Jacks Lab website http://web.mit.edu/ccr/labs/jacks/protocols/pSico.html