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Addgene

pCS2 xIGF1
(Plasmid #24967)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24967 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2
  • Backbone size w/o insert (bp) 4100
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    insulin-like growth factor 1
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    360
  • Entrez Gene
    igf1 (a.k.a. IGF-I, xigf1, igf1-A)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

mRNA injection sense: cut with NotI, transcribe with Sp6

Anti-sense in situ probe: cut with EcoRI, transcribe with T7

PCR fragment of IGF1 (EcoRI-XhoI) from stage 27 cDNA inserted into pCS2 (EcoRI-XhoI).

Sense: 5'AAA GAA TTC ATG GAA ACA AAC AAC AAT

Antisense: 5'-TTT CTC GAG TTA CAT TCG GAA TCC TCG

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2 xIGF1 was a gift from Edward De Robertis (Addgene plasmid # 24967 ; http://n2t.net/addgene:24967 ; RRID:Addgene_24967)

  • For your References section:

    Neural and head induction by insulin-like growth factor signals. Pera EM, Wessely O, Li SY, De Robertis EM. Dev Cell. 2001 Nov . 1(5):655-65. 10.1016/S1534-5807(01)00069-7 PubMed 11709186
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