pCDNA4-Myc-HisA-H248Y-Sirt3
(Plasmid #24917)

• Depositing Lab: Toren Finkel
• Publication: Ahn et al Proc Natl Acad Sci U S A. 2008 Sep 23. 105(38):14447-52.

Backbone

• Vector backbone: pcDNA4/myc-His A
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5075
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Sirt3
• Species: H. sapiens (human)
• Mutation: His248 mutated to Tyr(Y)
• Entrez Gene: SIRT3 (a.k.a. SIR2L3)
• Tags / Fusion Proteins:
  • Myc (C terminal on insert)
  • His (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Catalytically inactive mutant