DCGS-10
(Plasmid
#24752)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 24752 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBSK-
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 2958
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Vector typein situ probe template
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert name(sex determining region Y)-box 10
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Alt nameSox10
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Alt nameSRY-box10
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Alt nameDom
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SpeciesM. musculus (mouse)
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Insert Size (bp)1450
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Mutation3' end of Sox10 cDNA for in situ probe. Transcribe with T3 for sense; transcribe with T7 for antisense.
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GenBank IDBC002824
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Entrez GeneSox10 (a.k.a. Dom, Sox21, gt)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRV (unknown if destroyed)
- 3′ cloning site Xho1 (unknown if destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
DCGS-10 was a gift from William Pavan (Addgene plasmid # 24752 ; http://n2t.net/addgene:24752 ; RRID:Addgene_24752) -
For your References section:
Sox10 mutation disrupts neural crest development in Dom Hirschsprung mouse model. Southard-Smith EM, Kos L, Pavan WJ. Nat Genet. 1998 Jan . 18(1):60-4. 10.1038/ng0198-60 PubMed 9425902