pBS-mPax3
(Plasmid #16427)

• Depositing Lab: Jonathan Epstein
• Publication: Jon Epstein Lab Plasmids (unpublished)

Backbone

• Vector backbone: pBluescript KS
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 3000
• Vector type: In Situ Template

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Pax3
• Species: M. musculus (mouse)
• Insert Size (bp): 345
• Mutation: None
• Entrez Gene: Pax3 (a.k.a. Pax-3, Sp, Splchl2, splotch)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T3
• 3′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Modification of pBH3.2 Pax3 plasmid from Martyn Goulding, Max Plank Institute (Goulding MD, Chalepakis G, Deutsch U, Erselius JR, Gruss P.
Pax-3, a novel murine DNA binding protein expressed during early neurogenesis.
EMBO J. 1991 10(5):1135-47). Deleted internal SmaI fragment using SmaI in polylinker and SmaI in insert.

Antisense probes are generated by digesting with EcoRI or HindIII and transcribing with T7 RNA Polymerase.