GAG-PKB-pcDNA3
(Plasmid
#24651)
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 24651 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepcDNA3
- Backbone size w/o insert (bp) 5400
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePKB
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SpeciesH. sapiens (human)
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Entrez GeneAKT1 (a.k.a. AKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA)
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Tag
/ Fusion Protein
- GAG (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
P106S mutation in GAG and S2D, V45L, D46E, A50S, K112R, E117T, S126G, D127E mutations detected in PKB Addgene quality control sequence. Author reports that these mutations do not affect function.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GAG-PKB-pcDNA3 was a gift from Jim Woodgett (Addgene plasmid # 24651 ; http://n2t.net/addgene:24651 ; RRID:Addgene_24651) -
For your References section:
Protein kinase B regulates T lymphocyte survival, nuclear factor kappaB activation, and Bcl-X(L) levels in vivo. Jones RG, Parsons M, Bonnard M, Chan VS, Yeh WC, Woodgett JR, Ohashi PS. J Exp Med. 2000 May 15. 191(10):1721-34. 10.1084/jem.191.10.1721 PubMed 10811865
