pcDNA4-PKCZeta WT His tagged
(Plasmid #24609)

• Depositing Lab: Jeff Wrana
• Publication: Wang et al Science. 2003 Dec 5. 302(5651):1775-9.

Backbone

• Vector backbone: pcDNA4/TO
• Backbone size w/o insert (bp): 5077
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PKCZeta
• Alt name: PKC zeta
• Species: H. sapiens (human)
• Entrez Gene: PRKCZ (a.k.a. PKC-ZETA, PKC2)
• Tag / Fusion Protein:
  • His (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA