pAdEasy-mRFP-gsk3 beta S9A
(Plasmid #24521)

• Depositing Lab: Torsten Wittmann
• Publication: Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908.

Backbone

• Vector backbone: pAdEasy-1
• Backbone manufacturer: Stratagene, ATCC
• Backbone size w/o insert (bp): 33414
• Vector type: Mammalian Expression, Adenoviral

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: GSK3beta(S9A)
• Alt name: GSK3beta
• Species: H. sapiens (human)
• Insert Size (bp): 2000
• Mutation: S9A. Mutant version of GSK3 that lacks the inhibitory phosphorylation site.
• Entrez Gene: GSK3B
• Tag / Fusion Protein:
  • mRFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CMV-F, EGFP-C

Resource Information

• A portion of this plasmid was derived from a plasmid made by: cDNA obtained from Niels Galjart
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

mRFP-GSK3beta S9A was cloned into pShuttle-CMV between KpnI and NotI then recombined by homologous recombination into pAdEasy-1.

AdEasy is a registered trademark of the Johns Hopkins University.