pEGFP-CLASP2 512-650 9xS/A
(Plasmid #24410)

• Depositing Lab: Torsten Wittmann
• Publication: Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: CLASP2 (512-650) 9xS/A
• Alt name: CLASP2
• Species: H. sapiens (human)
• Insert Size (bp): 400
• Mutation: Nonphosphorylatable CLASP2 deletion mutant that contains only the MT plus end tracking activity. Mutation of six serines (S594/598/602/606/610/614) to alanine in GSK3β motif plus S568A, S572A, S576A.
• GenBank ID: NP_055912
• Entrez Gene: CLASP2
• Tag / Fusion Protein:
  • EGFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XmaI (unknown if destroyed)
• 3′ cloning site: MluI (unknown if destroyed)
• 5′ sequencing primer: EGFP-C

Resource Information

• Supplemental Documents:
  • CLASP2 Reference sequence.
• A portion of this plasmid was derived from a plasmid made by: CLASP2 cDNA was a gift from Anna Akhmanova

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

See the attached file for the old NIH reference sequence. Numbering starts at amino acid 78 and is off by 8 amino acids because of an insertion that is not accounted for.