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Addgene

pShuttle-EGFP-CLASP2 340-1362 9xS/A
(Plasmid #24373)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24373 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pShuttle-CMV
  • Backbone manufacturer
    Agilent Technologies
  • Backbone size w/o insert (bp) 7469
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CLASP2 (340-1362) 9xS/A
  • Alt name
    CLASP2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3800
  • Mutation
    Nonphosphorylatable CLASP2 deletion mutant. Mutation of serines in GSK3β motif between S594 to S614 plus S568/572/576A.
  • Entrez Gene
    CLASP2
  • Tag / Fusion Protein
    • EGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer CMV-F, EGFP-C
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    CLASP2 cDNA was a gift from Anna Akhmanova

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See the attached file for the old NIH reference sequence. Numbering starts at amino acid 78 and is off by 8 amino acids because of an insertion that is not accounted for.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pShuttle-EGFP-CLASP2 340-1362 9xS/A was a gift from Torsten Wittmann (Addgene plasmid # 24373 ; http://n2t.net/addgene:24373 ; RRID:Addgene_24373)
  • For your References section:

    GSK3beta phosphorylation modulates CLASP-microtubule association and lamella microtubule attachment. Kumar P, Lyle KS, Gierke S, Matov A, Danuser G, Wittmann T. J Cell Biol. 2009 Mar 23. 184(6):895-908. 10.1083/jcb.200901042 PubMed 19289791