ins-cre_cryaa-Venus
(Plasmid #24333)

• Depositing Lab: Didier Stainier
• Publication: Hesselson et al Proc Natl Acad Sci U S A. 2009 Sep 1. 106(35):14896-901.

Backbone

• Vector backbone: pBluescript
• Backbone size w/o insert (bp): 3000
• Vector type: Worm Expression, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Cre
• Entrez Gene: cre (a.k.a. P1_gp003)
• Tag / Fusion Protein:
  • Venus (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: Cre-R
• 3′ sequencing primer: EGFP-N

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

ins-Cre_cryaa-Venus was generated by placing the Cre coding sequence downstream of 1.5 kb of the proximal insulin promoter. The cryaa-Venus cassette was inserted downstream of Cre in the reverse orientation using 0.54 kb of the cryaa promoter.