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pAc-mCh-Tub
(Plasmid #24288)

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Item Catalog # Description Quantity Price (USD)
Plasmid 24288 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAc-mCherryW
  • Backbone size w/o insert (bp) 7952
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Alpha-Tubulin
  • Alt name
    Alpha-Tub84B
  • Alt name
    Alpha-Tubulin at 84B
  • Alt name
    CG1913
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    1353
  • GenBank ID
    NM_057424
  • Entrez Gene
    alphaTub84B (a.k.a. Dmel_CG1913, 1t, ALPHA 84C, Alpha-Tubulin84B, BEST:LD32507, CG1913, DM1alpha, DTA1, Dmel\CG1913, T, TBA1_DROME, Talpha1, Tub, Tub 84B, Tub1C, Tuba84B, Tubalpha1, a-tub84B, a1t, aTub84B, alpha-Tub, alpha-Tub84B, alpha-tub, alpha-tub84B, alpha-tubulin, alpha-tubulin 84B, alpha1, alpha1t, alpha1tub, alpha1tub84B, alpha84B, alphaT, alphaTUB, alphaTUB84B, alphaTub, alphaTub1, alphaTub84, alphat-1, alphatub, alphatub84B, anon-EST:Liang-1.59, anon-EST:Liang-2.30, chr3R:2914276..2914416, clone 1.59, clone 2.30, l(3)84Bd, l(3)g3, ms(3)nc33, nc33, tub, tubA84B, tubalpha1, tubulin)
  • Tag / Fusion Protein
    • mCherry (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AttR1 (unknown if destroyed)
  • 3′ cloning site AttR2 (unknown if destroyed)
  • 5′ sequencing primer RFP Fwd
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Tub84b cDNA was cloned, inserted into pENTR-D-Topo and the insert was transferred into pAC-mCherry-W using LR cloning.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAc-mCh-Tub was a gift from Ron Vale (Addgene plasmid # 24288 ; http://n2t.net/addgene:24288 ; RRID:Addgene_24288)

  • For your References section:

    Dynamics of myosin, microtubules, and Kinesin-6 at the cortex during cytokinesis in Drosophila S2 cells. Vale RD, Spudich JA, Griffis ER. J Cell Biol. 2009 Sep 7. 186(5):727-38. 10.1083/jcb.200902083 PubMed 19720876
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