pCDNA3 Flag DLC1
(Plasmid #24265)

• Depositing Lab: Roger Davis
• Publication: Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 100(5):2432-7.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: invitrogen
• Backbone size w/o insert (bp): 5446
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: DLC1
• Species: H. sapiens (human)
• Insert Size (bp): 305
• GenBank ID: NM_001037494
• Entrez Gene: DYNLL1 (a.k.a. DLC1, DLC8, DNCL1, DNCLC1, LC8, LC8a, PIN, hdlc1)
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Hind III (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry