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pFU-cMVIIA-PE
(Plasmid #24147)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24147 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFUGW
  • Backbone size w/o insert (bp) 9212
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Use low-recombination strains like Hb101 or Stbl3.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    loxP-DsRed-loxP-omega-Conotoxin MVIIA-FLAG-PDGF_R_TM-EGFP
  • Species
    H. sapiens (human); Conus magus, Aequorea victoria
  • Insert Size (bp)
    1912
  • Entrez Gene
    PDGFRB (a.k.a. CD140B, IBGC4, IMF1, JTK12, KOGS, PDGFR, PDGFR-1, PDGFR1, PENTT)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer GTCCGCTAAATTCTGGC
  • 3′ sequencing primer GCCATACGGGAAGCAATAG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Cre-recombinase dependent lentiviral vector.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFU-cMVIIA-PE was a gift from Ines Ibanez-Tallon (Addgene plasmid # 24147 ; http://n2t.net/addgene:24147 ; RRID:Addgene_24147)

  • For your References section:

    Silencing neurotransmission with membrane-tethered toxins. Auer S, Sturzebecher AS, Juttner R, Santos-Torres J, Hanack C, Frahm S, Liehl B, Ibanez-Tallon I. Nat Methods. 2010 Feb 7. ():. 10.1038/nmeth.1425 PubMed 20139968
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