pFU-MVIIA-PE
(Plasmid
#24144)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 24144 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepFUGW
- Backbone size w/o insert (bp) 9218
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Growth instructionsUse low-recombination strains like Hb101 or Stbl3.
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameomega-Conotoxin MVIIA-FLAG-PDGF_R_TM-EGFP
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SpeciesH. sapiens (human); Conus magus, Aequorea victoria
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Insert Size (bp)1110
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Entrez GenePDGFRB (a.k.a. CD140B, IBGC4, IMF1, JTK12, KOGS, PDGFR, PDGFR-1, PDGFR1, PENTT)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer GTCCGCTAAATTCTGGC
- 3′ sequencing primer GCCATACGGGAAGCAATAG (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFU-MVIIA-PE was a gift from Ines Ibanez-Tallon (Addgene plasmid # 24144 ; http://n2t.net/addgene:24144 ; RRID:Addgene_24144) -
For your References section:
Silencing neurotransmission with membrane-tethered toxins. Auer S, Sturzebecher AS, Juttner R, Santos-Torres J, Hanack C, Frahm S, Liehl B, Ibanez-Tallon I. Nat Methods. 2010 Feb 7. ():. 10.1038/nmeth.1425 PubMed 20139968