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Addgene

mCD4 e/p Sal-(hCD2)
(Plasmid #24070)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24070 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    mCD4 e/p
  • Backbone size w/o insert (bp) 10200
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CD2
  • Species
    H. sapiens (human)
  • Entrez Gene
    CD2 (a.k.a. LFA-2, SRBC, T11)
  • Tag / Fusion Protein
    • mCD 4 promoter (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (destroyed during cloning)
  • 3′ cloning site Asp718 (unknown if destroyed)
  • 5′ sequencing primer hCD2-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mCD4 e/p Sal-(hCD2) was a gift from Dan Littman (Addgene plasmid # 24070 ; http://n2t.net/addgene:24070 ; RRID:Addgene_24070)

  • For your References section:

    A lineage-specific transcriptional silencer regulates CD4 gene expression during T lymphocyte development. Sawada S, Scarborough JD, Killeen N, Littman DR. Cell. 1994 Jun 17. 77(6):917-29. 10.1016/0092-8674(94)90140-6 PubMed 8004678
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