pEM3 = ncs-1p::nCre
(Plasmid #24034)

• Depositing Lab: Cori Bargmann
• Publication: Macosko et al Nature. 2009 Apr 30. 458(7242):1171-5.

Backbone

• Vector backbone: Itself
• Backbone size w/o insert (bp): 7595
• Vector type: Worm Expression, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: ncs-1
• Alt name: ncs-1 promoter
• Species: C. elegans (nematode)
• Insert Size (bp): 7595
• Entrez Gene: ncs-1
• Tag / Fusion Protein:
  • nCre (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: NCS-1 promoter:

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry