pEM1 = flp-21::LoxPStopLoxP::npr-1 SL2 GFP
(Plasmid #24033)

• Depositing Lab: Cori Bargmann
• Publication: Macosko et al Nature. 2009 Apr 30. 458(7242):1171-5.

Backbone

• Vector backbone: flp21::loxPStopLoxP
• Backbone size w/o insert (bp): 10000
• Vector type: Worm Expression, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Npr1
• Species: C. elegans (nematode)
• Entrez Gene: npr-1 (a.k.a. CELE_C39E6.6)
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (unknown if destroyed)
• 3′ cloning site: KpnI (unknown if destroyed)
• 5′ sequencing primer: flp-21 promoter
• 3′ sequencing primer: GFP-R

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry