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Addgene

pyrFEKO-BLE
(Plasmid #24023)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24023 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pyrFEKO
  • Backbone size (bp) 5529
  • Vector type
    Floxed targeting vector for Trypanosome
  • Selectable markers
    Phleomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    None

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI/PvuII (not destroyed)
  • 3′ cloning site SbfI/HindIII (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Unpublished (variant of constructs described in Scahill et al 2008).

For more information about using this vector, please see: http://tryps.rockefeller.edu/trypsru2_cre-lox.html

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pyrFEKO-BLE was a gift from George Cross (Addgene plasmid # 24023 ; http://n2t.net/addgene:24023 ; RRID:Addgene_24023)
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