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Addgene

pCO57
(Plasmid #24015)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24015 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pLEW82
  • Backbone manufacturer
    George Cross
  • Backbone size (bp) 5000
  • Vector type
    Trypanosome Expression
  • Selectable markers
    Phleomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    None
  • Tag / Fusion Protein
    • EGFP (C terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Target locus: rRNA spacer

Regulation: 2 tet Op 4 T7 terminators

For additional information see George Cross's website at http://tryps.rockefeller.edu/trypsru2_plasmids.html

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCO57 was a gift from George Cross (Addgene plasmid # 24015 ; http://n2t.net/addgene:24015 ; RRID:Addgene_24015)