pUB39
(Plasmid
#26300)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 26300 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLEW82
- Backbone size w/o insert (bp) 4916
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Vector typeTrypanosome inducible expression vector
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Selectable markersZeocin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameVSG117 (Lister 427-4) cDNA
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Alt namepLEW82
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Alt nameVariant Surface Glycoprotein (VSG)
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SpeciesTrypanosoma brucei
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Insert Size (bp)1582
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GenBank IDV01387
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site BamH1 (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
2002: Mutational analysis of the variant surface glycoprotein GPI-anchor signal sequence in Trypanosoma brucei. Boehme U & Cross GAM. J Cell Sci 115:805-16
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUB39 was a gift from George Cross (Addgene plasmid # 26300 ; http://n2t.net/addgene:26300 ; RRID:Addgene_26300)