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Addgene

pUB39
(Plasmid #26300)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26300 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pLEW82
  • Backbone size w/o insert (bp) 4916
  • Vector type
    Trypanosome inducible expression vector
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    VSG117 (Lister 427-4) cDNA
  • Alt name
    pLEW82
  • Alt name
    Variant Surface Glycoprotein (VSG)
  • Species
    Trypanosoma brucei
  • Insert Size (bp)
    1582
  • GenBank ID
    V01387

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site BamH1 (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

2002: Mutational analysis of the variant surface glycoprotein GPI-anchor signal sequence in Trypanosoma brucei. Boehme U & Cross GAM. J Cell Sci 115:805-16

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUB39 was a gift from George Cross (Addgene plasmid # 26300 ; http://n2t.net/addgene:26300 ; RRID:Addgene_26300)