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Addgene

pRAM24
(Plasmid #234313)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 234313 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBBR1
  • Backbone size w/o insert (bp) 2820
  • Total vector size (bp) 4007
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    U1367 Targeting RAM, Barcode 1
  • Species
    Synthetic
  • Insert Size (bp)
    1112
  • Promoter P. CymRC

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.04.16.536800 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRAM24 was a gift from James Chappell (Addgene plasmid # 234313 ; http://n2t.net/addgene:234313 ; RRID:Addgene_234313)

  • For your References section:

    Information storage across a microbial community using universal RNA barcoding. Kalvapalle PB, Staubus A, Dysart MJ, Gambill L, Reyes Gamas K, Lu LC, Silberg JJ, Stadler LB, Chappell J. Nat Biotechnol. 2025 Mar 18. doi: 10.1038/s41587-025-02593-0. 10.1038/s41587-025-02593-0 PubMed 40102641
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