pLM-mCherry-Klf4
(Plasmid #23243)

• Depositing Lab: Michel Sadelain
• Publication: Papapetrou et al Proc Natl Acad Sci U S A. 2009 Aug 4. 106(31):12759-64.

Backbone

• Vector backbone: N/A
• Backbone size w/o insert (bp): 6655
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mCherry_2A_KLF4
• Alt name: KLF4
• Species: H. sapiens (human); synthetic fluorescent protein
• Insert Size (bp): 2193
• Entrez Gene: KLF4 (a.k.a. EZF, GKLF)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: hPGK-F (5'GTGTTCCGCATTCTGCAAG-3')
• 3′ sequencing primer: WPRE-R (5'-CATAGCGTAAAAGGAGCAACA-3')

Resource Information

• Articles Citing this Plasmid:
  • 9 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry