loxP-Hygro-loxP-HA-mStayGold-Rab11 HR
(Plasmid
#229678)
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PurposeHomology repair plasmid for endogenous tagging of Rab11 at the N-terminus with monomeric StayGold and an HA epitope tag. Contains a hygromycin resistance cassette for selection of edited cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 229678 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTWIST Amp high copy
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Backbone manufacturerTWIST
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Vector typeCRISPR
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
loxP-Hygro-loxP-HA-mStayGold-Rab11 HR was a gift from Francesca Bottanelli (Addgene plasmid # 229678 ; http://n2t.net/addgene:229678 ; RRID:Addgene_229678) -
For your References section:
ARF1 compartments direct cargo flow via maturation into recycling endosomes. Stockhammer A, Adarska P, Natalia V, Heuhsen A, Klemt A, Bregu G, Harel S, Rodilla-Ramirez C, Spalt C, Ozsoy E, Leupold P, Grindel A, Fox E, Mejedo JO, Zehtabian A, Ewers H, Puchkov D, Haucke V, Bottanelli F. Nat Cell Biol. 2024 Oct 4. doi: 10.1038/s41556-024-01518-4. 10.1038/s41556-024-01518-4 PubMed 39367144