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Addgene

loxP-G418-loxP-3xALFA-Halo-Rab11 HR
(Plasmid #229676)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 229676 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTWIST Amp high copy
  • Backbone manufacturer
    TWIST
  • Vector type
    CRISPR
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    genomic sequence Rab11 locus
  • Species
    H. sapiens (human)
  • Entrez Gene
    RAB11A (a.k.a. YL8)
  • Tag / Fusion Protein
    • Halo, 3xALFA

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    loxP-G418-loxP-3xALFA-Halo-Rab11 HR was a gift from Francesca Bottanelli (Addgene plasmid # 229676 ; http://n2t.net/addgene:229676 ; RRID:Addgene_229676)

  • For your References section:

    ARF1 compartments direct cargo flow via maturation into recycling endosomes. Stockhammer A, Adarska P, Natalia V, Heuhsen A, Klemt A, Bregu G, Harel S, Rodilla-Ramirez C, Spalt C, Ozsoy E, Leupold P, Grindel A, Fox E, Mejedo JO, Zehtabian A, Ewers H, Puchkov D, Haucke V, Bottanelli F. Nat Cell Biol. 2024 Oct 4. doi: 10.1038/s41556-024-01518-4. 10.1038/s41556-024-01518-4 PubMed 39367144
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