pTec_mCherry
(Plasmid
#229145)
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PurposePromoter strength evaluation of Ptec
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 229145 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLp_3050sNuc
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Backbone manufacturerAddgene
- Backbone size w/o insert (bp) 2800
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Erythromycin, 200 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemCherry
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SpeciesDiscosoma spp
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Insert Size (bp)711
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MutationNo
- Promoter Ptec
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTec_mCherry was a gift from Shrikrishnan Sankaran (Addgene plasmid # 229145 ; http://n2t.net/addgene:229145 ; RRID:Addgene_229145) -
For your References section:
Discovery of a high-performance phage-derived promoter/repressor system for probiotic lactobacillus engineering. Blanch-Asensio M, Tadimarri VS, Wilk A, Sankaran S. Microb Cell Fact. 2024 Feb 7;23(1):42. doi: 10.1186/s12934-024-02302-7. 10.1186/s12934-024-02302-7 PubMed 38326819