-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 22910 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepAAV
- Backbone size w/o insert (bp) 4000
-
Vector typeMammalian Expression, AAV ; Adeno-associated virus
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameChicken Beta-actin promoter driving DsRedExpress
-
Insert Size (bp)3400
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer Unknown
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Resource Information
-
Articles Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
There are a few sequence mismatches with Chicken beta-actin promoter in BLAST, however this plasmid is functions as described in the associated publication.
Please note that this plasmid may have an alternative left ITR.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pAAV-CAG-RFP was a gift from Edward Callaway (Addgene plasmid # 22910 ; http://n2t.net/addgene:22910 ; RRID:Addgene_22910) -
For your References section:
Preferential labeling of inhibitory and excitatory cortical neurons by endogenous tropism of adeno-associated virus and lentivirus vectors. Nathanson JL, Yanagawa Y, Obata K, Callaway EM. Neuroscience. 2009 Jun 30. 161(2):441-50. 10.1016/j.neuroscience.2009.03.032 PubMed 19318117
