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Addgene

pSYFP2-SCFP3A
(Plasmid #22905)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22905 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4000
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SYFP2-SCFP3A
  • Alt name
    mVenus(L68V)-SCFP3A
  • Species
    A. victoria
  • Insert Size (bp)
    1500

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSYFP2-SCFP3A was a gift from Dorus Gadella (Addgene plasmid # 22905 ; http://n2t.net/addgene:22905 ; RRID:Addgene_22905)

  • For your References section:

    Cyan and yellow super fluorescent proteins with improved brightness, protein folding, and FRET Foerster radius. Kremers GJ, Goedhart J, van Munster EB, Gadella TW. Biochemistry. 2006 May 30. 45(21):6570-80. 10.1021/bi0516273 PubMed 16716067
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