pInducer20_EGFP
(Plasmid
#228878)
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PurposeTet-inducible lentiviral vector for expression of optimized GFP
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 228878 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepInducer20
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Backbone manufacturerAddgene #44012
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Vector typeLentiviral
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameEGFP
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer LNCX_primer (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byEGFP cloned from pENTR1A-GFP-N2 (FR1) Addgene #19364
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Control vector for expression of EGFP
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pInducer20_EGFP was a gift from Ramiro Iglesias-Bartolome (Addgene plasmid # 228878 ; http://n2t.net/addgene:228878 ; RRID:Addgene_228878) -
For your References section:
An improved TEAD dominant-negative protein inhibitor to study Hippo YAP1/TAZ-dependent transcription. Branch B, Yuan Y, Cascone M, Raimondi F, Iglesias-Bartolome R. bioRxiv [Preprint]. 2024 Oct 3:2024.10.03.615022. doi: 10.1101/2024.10.03.615022. 10.1101/2024.10.03.615022 PubMed 39502361